GB Script™ III 1 st Strand cDNA Synthesis Kit (+ gDNA wiper)
This product delivers high efficiency of first strand cDNA synthesis. The 5× gDNA wiper Mix in the kit can quickly remove genomic DNA contamination at 42℃ for 2 min, which makes the res µLts more reliable and simplifies qPCR primer design process without the need to design primers across introns. The kit contains two separated primers : the reverse transcription primers Oligo (dT) 20 VN and Random hexamers, allowing users to choose primers flexibly for subsequent experiments as needed. The kit can syntheVolume f µLl-length cDNA (up to 20 kb) for downstream experiments such as cloning, and can also syntheVolume a highly uniform cDNA for qPCR quantification.
Components
R312-01 50rxn (20μl/rxn)
R312-02 100rxn (20μl/rxn)
RNasefreeddH2O
1 ml
1 ml
5× gDNAwiperMix
100 μl
200 μl
10x RTMix
100 μl
200 μl
GB-Script™ III EnzymeMix
100 μl
200 μl
Oligo (dT)20VN
50 μl
100 μl
RandomHexamers
50 μl
100 μl