FlyCut® PvuI
Product Details
FlyCut® PvuI is expressed and purified from E.coli that carries the recombinant PvuI gene. The molec µLar weight is 30.8 kDa, with the recognition site at CGAT^CG The reaction is conducted for 5-15 minutes at 37℃, and heat-inactivated at 80℃ for 20 minutes. This enzyme is not sensitive to dam, dcm, but sensitive to mammalian CpG methylation.
Recognition Site
5’...CGAT↓CG...3’
3’...GC↑TAGC...5’
Applications
Genomic DNA, plasmid DNA, PCR product
Storage
at -20°C for two years
Shipping
Dry ice (-70 ℃)
Product Contents
Component JP201-01 JP201-02
FlyCut® PvuI 250 units 2×250 units
10×FlyCut® Buffer 250 μl 250 μl
10×DNA Loading Buffer 1 ml 1 ml