FlyCut® EcoRV

Product Details
FlyCut® EcoRV is expressed and purified from E.coli that carries the recombinant EcoRV gene. The molec µLar weight is 29.5 kDa, with the recognition site at GAT^ATC. The reaction is conducted for 5-15 minutes at 37℃, and heat-inactivated at 65℃ for 20 minutes. This enzyme is not sensitive to dam or dcm methylation, but sensitive to mammalian CpG methylation.

Recognition Site
5’...GAT↓ATC...3’
3’...CTA↑TAG...5’

Applications
Genomic DNA, plasmid DNA, PCR product

Storage
at -20°C for two years

Shipping
Dry ice (-70 ℃)


Product Contents

Component JE301-01 JE301-02
FlyCut® EcoRV 2,000 units 2×2,000 units
10×FlyCut® Buffer 500 μl 1 ml
10×DNA Loading Buffer 1 ml 1 ml