CASP3 antibody

Volume : 100 µg
Purification : protein A+G purified
Form : liquid
Purity : 95% as determined by SDS-PAGE
Host : Mouse
Clonality : monoclonal
Clone ID : 2G7
Isotype : IgG1
Storage : 0.01MPBS pH 7.4,-20? for 12 months(Avoid repeated freeze / thaw cycles.)
Background : Caspase 3, also named as CPP32, SCA-1 and Apopain, belongs to the peptidase C14A family. Caspase 3 is involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase(PARP) at a '216-Asp-|-Gly-217' bond. Caspase 3 cleaves and activates sterol reg µLatory element binding proteins(SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6,-7 and-9. Caspase 3 is involved in the cleavage of huntingtin. Caspase 3 can also form heterocomplex with other proteins and performs MW of 50-70 kDa. This antibody can recognize p17, p19 and p32 of Caspase 3.
Immunogen : caspase 3, apoptosis-related cysteine peptidase
Aliases : Apopain, CASP 3, CASP3, Caspase 3, Caspase 3/p17, Caspase3, CPP 32, CPP32, CPP32B, Cysteine protease CPP32, Protein Yama, SCA 1 SREBP cleavage activity 1
Observed MW : 32-35 kDa, 19 kDa, 17 kDa
Uniprot ID : P42574
Reactivity : Human, Mouse, Rat
Application : ELISA, IHC, WB,IF
Recommended dilution : WB : 1 : 500-1 : 2000; IHC : 1 : 50-1 : 300; IF : 1 : 50-1 : 200
Gene ID : 836
Research Area : Cancer, Metabolism, Neuroscience