MAGOH antibody

Volume : 100 µg
Purification : Immunogen affinity purified
Form : liquid
Purity : 95% as determined by SDS-PAGE
Host : Rabbit
Clonality : polyclonal Ab
Clone ID :
Isotype : IgG
Storage : PBS with 0.02% sodium azide and 50% glycerol pH 7.3,-20? for 12 months(Avoid repeated freeze / thaw cycles.)
Background : Core component of the splicing-dependent m µLtiprotein exon junction complex(EJC) deposited at splice junctions on mRNAs. The EJC is a dynamic structure consisting of core proteins and several peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. The EJC marks the position of the exon-exon junction in the mature mRNA for the gene expression machinery and the core components remain bound to spliced mRNAs thro µghout all stages of mRNA metabolism thereby influencing downstream processes including nuclear mRNA export, subcell µLar mRNA localization, translation efficiency and nonsense-mediated mRNA decay(NMD). The MAGOH-RBM8A heterodimer inhibits the ATPase activity of EIF4A3, thereby trapping the ATP-bound EJC core onto spliced mRNA in a stable conformation. The MAGOH-RBM8A heterodimer interacts with the EJC key reg µLator PYM1 leading to EJC disassembly in the cytoplasm and translation enhancement of EJC-bearing spliced mRNAs by recruiting them to the ribosomal 48S preinitiation complex. Involved in the splicing mod µLation of BCL2L1/Bcl-X(and probably other apoptotic genes); specifically inhibits formation of proapoptotic isoforms such as Bcl-X(S); the function is different from the established EJC assembly.
Immunogen : mago-nashi homolog, proliferation-associated(Drosophila)
Aliases : MAGOHA
Observed MW : 17 kDa
Uniprot ID : P61326
Reactivity : Human
Application : ELISA, IHC, IP, WB
Recommended dilution : WB : 1 : 500-1 : 2000; IP : 1 : 200-1 : 1000; IHC : 1 : 20-1 : 200
Gene ID : 4116
Research Area : Neuroscience, Stem Cells, Metabolism, Developmental biology