c Kit/CD117 antibody

Volume : 100 µg
Purification : Immunogen affinity purified
Form : liquid
Purity : 95% as determined by SDS-PAGE
Host : Rabbit
Clonality : polyclonal Ab
Clone ID :
Isotype : IgG
Storage : PBS with 0.02% sodium azide and 50% glycerol pH 7.3,-20? for 12 months(Avoid repeated freeze / thaw cycles.)
Background : Tyrosine-protein kinase that acts as cell-surface receptor for the cytokine KITLG/SCF and plays an essential role in the reg µLation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. In response to KITLG/SCF binding, KIT can activate several signaling pathways. Phosphorylates PIK3R1 PLCG1 SH2B2/APS and CBL. Activates the AKT1 signaling pathway by phosphorylation of PIK3R1 the reg µLatory subunit of phosphatidylinositol 3-kinase. Activated KIT also transmits signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. Promotes activation of STAT family members STAT1 STAT3, STAT5A and STAT5B. Activation of PLCG1 leads to the production of the cell µLar signaling molec µLes diacylglycerol and inositol 14,5-trisphosphate. KIT signaling is mod µLated by protein phosphatases, and by rapid internalization and degradation of the receptor. Activated KIT promotes phosphorylation of the protein phosphatases PTPN6/SHP-1 and PTPRU, and of the transcription factors STAT1 STAT3, STAT5A and STAT5B. Promotes phosphorylation of PIK3R1 CBL, CRK(isoform Crk-II), LYN, MAPK1/ERK2 and/or MAPK3/ERK1 PLCG1 SRC and SHC1.
Immunogen : Mast/stem cell growth factor receptor Kit
Aliases : C Kit, CD117, KIT, PBT, Proto oncogene c Kit, SCFR, Tyrosine protein kinase Kit
Observed MW : 140 kDa
Uniprot ID : P10721
Reactivity : Human
Application : ELISA, WB, IHC, FC,IF
Recommended dilution : WB : 1 : 200-1 : 1000; IHC : 1 : 50-1 : 500; IF : 1 : 50-1 : 500
Gene ID :
Research Area : Signal Transduction, Metabolism, Immunology, Cancer, Developmental biology, Stem Cells