MAPKAPK2 (Ab 272) Antibody

Volume : 100 µL
Clone Number :
Aliases : MAP kinase activated protein Kinase 2 antibody; MAP kinase-activated protein kinase 2 antibody; MAPK activated protein kinase 2 antibody; MAPK-activated protein kinase 2 antibody; MAPK2_HUMAN antibody; MAPKAP K2 antibody; MAPKAP kinase 2 antibody; MAPKAPK 2 antibody; MAPKAPK-2 antibody; MAPKAPK2 antibody; Mitogen activated protein kinase activated protein kinase 2 antibody; MK 2 antibody; MK2 antibody
Product Type : polyclonal Ab Antibody
Immunogen Species : Homo sapiens (Human)
UniProt ID : P49137
Immunogen : SyntheVolumed non-phosphopeptide derived from Human MAPKAPK2 around the phosphorylation site of serine 272 (A-I-S(p)-P-G).
Raised in : Rabbit
Species Reactivity : Human, Mouse
Tested Applications : ELISA, WB, IHC; WB : 1 : 500-1 : 3000, IHC : 1 : 50-1 : 100
Background : Stress-activated serine/threonine-protein kinase involved in cytokines production, endocytosis, reorganization of the cytoskeleton, cell migration, cell cycle control, chromatin remodeling, DNA damage response and transcriptional reg µLation. Following stress, it is phosphorylated and activated by MAP kinase p38-alpha/MAPK14, leading to phosphorylation of substrates. Phosphorylates serine in the peptide sequence, Hyd-X-R-X(2)-S, where Hyd is a large hydrophobic residue. Phosphorylates ALOX5, CDC25B, CDC25C, ELAVL1 HNRNPA0, HSF1 HSP27/HSPB1 KRT18, KRT20, LIMK1 LSP1 PABPC1 PARN, PDE4A, RCSD1 RPS6KA3, TAB3 and TTP/ZFP36. Mediates phosphorylation of HSP27/HSPB1 in response to stress, leading to dissociate HSP27/HSPB1 from large small heat-shock protein (sHsps) oligomers and impair their chaperone activities and ability to protect against oxidative stress effectively. Involved in inflammatory response by reg µLating tumor necrosis factor (TNF) and IL6 production post-transcriptionally : acts by phosphorylating AU-rich elements (AREs)-binding proteins ELAVL1 HNRNPA0, PABPC1 and TTP/ZFP36, leading to reg µLate the stability and translation of TNF and IL6 mRNAs. Phosphorylation of TTP/ZFP36, a major post-transcriptional reg µLator of TNF, promotes its binding to 14-3-3 proteins and reduces its ARE mRNA affinity leading to inhibition of dependent degradation of ARE-containing transcript. Also involved in late G2/M checkpoint following DNA damage thro µgh a process of post-transcriptional mRNA stabilization : following DNA damage, relocalizes from nucleus to cytoplasm and phosphorylates HNRNPA0 and PARN, leading to stabilize GADD45A mRNA. Involved in toll-like receptor signaling pathway (TLR) in dendritic cells : required for acute TLR-induced macropinocytosis by phosphorylating and activating RPS6KA3.

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Stokoe D., Biochem. J. 296 : 843-849(1993).
Coxon P.Y., Cell. Signal. 15 : 993-1001(2003).
Clonality : polyclonal Ab
Isotype : IgG
Purification Method : The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Conj µgate : Non-conj µgated
Buffer : Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form : liquid
Stroage : Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Target Names : MAPKAPK2
Research Areas : Cardiovasc µLar;Signal transduction