L Antibody HRP conjugated

Volume : 50 µL
Clone Number :
Aliases : L antibody; RNA-directed RNA polymerase L antibody; Protein L antibody; Large structural protein antibody; Replicase antibody; Transcriptase) [Includes : RNA-directed RNA polymerase antibody; EC 2.7.7.48); mRNA antibody; guanine-N(7)-)-methyltransferase antibody; EC 2.1.1.56); GDP polyribonucleotidyltransferase antibody; EC 2.7.7.88); Cap-specific mRNA antibody; nucleoside-2'-O-)-methyltransferase 2 antibody; EC 2.1.1.296)] antibody
Product Type : polyclonal Ab Antibody
Immunogen Species : Vesic µLar stomatitis Indiana virus
UniProt ID : Q8B0H0
Immunogen : Recombinant Vesic µLar stomatitis Indiana virus RNA-directed RNA polymerase L protein (598-784AA)
Raised in : Rabbit
Species Reactivity : Vesic µLar stomatitis Indiana virus
Tested Applications : ELISA
Background : RNA-directed RNA polymerase that catalyzes the transcription of viral mRNAs, their capping and polyadenylation. The template is composed of the viral RNA tightly encapsidated by the nucleoprotein (N). The viral polymerase binds to the genomic RNA at the 3\' leader promoter, and transcribes subsequently all viral mRNAs with a decreasing efficiency. The first gene is the most transcribed, and the last the least transcribed. The viral phosphoprotein acts as a processivity factor. Capping is concommitant with initiation of mRNA transcription. Indeed, a GDP polyribonucleotidyl transferase (PRNTase) adds the cap structure when the nascent RNA chain length has reached few nucleotides. Ribose 2\'-O methylation of viral mRNA cap precedes and facilitates subsequent guanine-N-7 methylation, both acticities being carried by the viral polymerase. Polyadenylation of mRNAs occur by a stuttering mechanism at a slipery stop site present at the end viral genes. After finishing transcription of a mRNA, the polymerase can resume transcription of the downstream gene.
Clonality : polyclonal Ab
Isotype : IgG
Purification Method : >95%, Protein G purified
Conj µgate : HRP
Buffer : Preservative : 0.03% Proclin 300
Constituents : 50% Glycerol, 0.01M PBS, pH 7.4
Form : Liquid
Stroage : Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Target Names : L
Research Areas : N/A