MAPK1/MAPK3 (Ab 205/222) Antibody

Volume : 100 µL
Clone Number :
Aliases : ERK 2 antibody; ERK-2 antibody; ERT1 antibody; Extracell µLar Signal Reg µLated Kinase 2 antibody; Extracell µLar signal-reg µLated kinase 2 antibody; MAP kinase 1 antibody; MAP kinase 2 antibody; MAP kinase isoform p42 antibody; MAPK 1 antibody; MAPK 2 antibody; Mapk1 antibody; MAPK2 antibody; Mitogen-activated protein kinase 1 antibody; Mitogen-activated protein kinase 2 antibody; MK01_HUMAN antibody; P38 antibody; P40 antibody; P41 antibody; p42-MAPK antibody; P42MAPK antibody; PRKM1 antibody; PRKM2 antibody; protein kinase, mitogen-activated, 1 antibody; protein kinase, mitogen-activated, 2 antibody; protein tyrosine kinase ERK2 antibody
Product Type : polyclonal Ab Antibody
Immunogen Species : Homo sapiens (Human)
UniProt ID : P28482
Immunogen : SyntheVolumed peptide derived from Internal of Human MAPK1.
Raised in : Rabbit
Species Reactivity : Human, Mouse, Rat
Tested Applications : ELISA, WB; WB : 1 : 500-1 : 3000
Background :
Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK1/ERK2 and MAPK3/ERK1 are the 2 MAPKs which play an important role in the MAPK/ERK cascade. They participate also in a signaling cascade initiated by activated KIT and KITLG/SCF. Depending on the cell µLar context, the MAPK/ERK cascade mediates diverse biological functions such as cell growth, adhesion, survival and differentiation thro µgh the reg µLation of transcription, translation, cytoskeletal rearrangements. The MAPK/ERK cascade plays also a role in initiation and reg µLation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors. About 160 substrates have already been discovered for ERKs. Many of these substrates are localized in the nucleus, and seem to participate in the reg µLation of transcription upon stim µLation. However, other substrates are found in the cytosol as well as in other cell µLar organelles, and those are responsible for processes such as translation, mitosis and apoptosis. Moreover, the MAPK/ERK cascade is also involved in the reg µLation of the endosomal dynamics, including lysosome processing and endosome cycling thro µgh the perinuclear recycling compartment (PNRC); as well as in the fragmentation of the Golgi apparatus during mitosis. The substrates include transcription factors (such as ATF2, BCL6, ELK1 ERF, FOS, HSF4 or SPZ1), cytoskeletal elements (such as CANX, CTTN, GJA1 MAP2, MAPT, PXN, SORBS3 or STMN1), reg µLators of apoptosis (such as BAD, BTG2, CASP9, DAPK1 IER3, MCL1 or PPARG), reg µLators of translation (such as EIF4EBP1) and a variety of other signaling-related molec µLes (like ARHGEF2, DCC, FRS2 or GRB10). Protein kinases (such as RAF1 RPS6KA1/RSK1 RPS6KA3/RSK2, RPS6KA2/RSK3, RPS6KA6/RSK4, SYK, MKNK1/MNK1 MKNK2/MNK2, RPS6KA5/MSK1 RPS6KA4/MSK2, MAPKAPK3 or MAPKAPK5) and phosphatases (such as DUSP1 DUSP4, DUSP6 or DUSP16) are other substrates which enable the propagation the MAPK/ERK signal to additional cytosolic and nuclear targets, thereby extending the specificity of the cascade. Mediates phosphorylation of TPR in respons to EGF stim µLation. May play a role in the spindle assembly checkpoint. Phosphorylates PML and promotes its interaction with PIN1 leading to PML degradation. Acts as a transcriptional repressor. Binds to a [GC]AAA[GC] consensus sequence. Repress the expression of interferon gamma-induced genes. Seems to bind to the promoter of CCL5, DMP1 IFIH1 IFITM1 IRF7, IRF9, LAMP3, OAS1 OAS2, OAS3 and STAT1. Transcriptional activity is independent of kinase activity.

Owaki H., Biochem. Biophys. Res. Commun. 182 : 1416-1422(1992).
Gonzalez F.A., FEBS Lett. 304 : 170-178(1992).
Dunham I., Nature 402 : 489-495(1999).
Clonality : polyclonal Ab
Isotype : IgG
Purification Method : The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Conj µgate : Non-conj µgated
Buffer : Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form : liquid
Stroage : Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Target Names : MAPK1/MAPK3
Research Areas : Neuroscience;Signal transduction?Stem cells