CARM1 (Ab 228) Antibody

Volume : 100 µL
Clone Number :
Aliases : carm1 antibody; CARM1_HUMAN antibody; Coactivator associated arginine methyltransferase 1 antibody; Coactivator-associated arginine methyltransferase 1 antibody; Histone arginine methyltransferase CARM 1 antibody; Histone arginine methyltransferase CARM1 antibody; Histone-arginine methyltransferase CARM1 antibody; PRMT 4 antibody; PRMT4 antibody; Protein arginine methyltransferase antibody; Protein arginine N methyltransferase 4 antibody; Protein arginine N-methyltransferase 4 antibody
Product Type : polyclonal Ab Antibody
Immunogen Species : Homo sapiens (Human)
UniProt ID : Q86X55
Immunogen : Peptide sequence around aa.226~230 (V-K-S-N-N) derived from CARM1
Raised in : Rabbit
Species Reactivity : Human, Mouse, Rat
Tested Applications : ELISA, WB, IF; WB : 1 : 500-1 : 1000, IF : 1 : 100-1 : 200
Background : Methylates (mono- and asymmetric dimethylation) the guanidino nitrogens of arginyl residues in several proteins involved in DNA packaging, transcription reg µLation, and mRNA stability. Recruited to promoters upon gene activation together with histone acetyltransferases from EP300/P300 and p160 families, methylates histone H3 at 'Arg-17' and activates transcription via chromatin remodeling. During nuclear hormone receptor activation and TCF7L2/TCF4 activation, acts synergically with EP300/P300 and either one of the p160 histone acetyltransferases NCOA1/SRC1 NCOA2/GRIP1 and NCOA3/ACTR or CTNNB1/beta-catenin to activate transcription. During myogenic transcriptional activation, acts together with NCOA3/ACTR as a coactivator for MEF2C. During monocyte inflammatory stim µLation, acts together with EP300/P300 as a coactivator for NF-kappa-B. Also seems to be involved in p53/TP53 transcriptional activation. Methylates EP300/P300, both at 'Arg-2142', which may loosen its interaction with NCOA2/GRIP1 and at 'Arg-580' and 'Arg-604' in the KIX domain, which impairs its interaction with CREB and inhibits CREB-dependent transcriptional activation. Also methylates arginine residues in RNA-binding proteins PABPC1 ELAVL1 and ELAV4, which may affect their mRNA-stabilizing properties and the half-life of their target mRNAs.

Selma El Messaoudi, et al. (2006) Proc Natl Acad Sci U S A; 103(36) : 13351
Clonality : polyclonal Ab
Isotype : IgG
Purification Method : Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conj µgates. Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Conj µgate : Non-conj µgated
Buffer : Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form : liquid
Stroage : Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Target Names : CARM1
Research Areas : Epigenetics and Nuclear Signaling;Cancer;Cell biology;Metabolism;Signal transduction