MAPK14 Antibody Biotin conjugated

Volume : 50 µg
Clone Number :
Aliases : CSAID Binding Protein 1 antibody; CSAID binding protein antibody; CSAID-binding protein antibody; Csaids binding protein antibody; CSBP 1 antibody; CSBP 2 antibody; CSBP antibody; CSBP1 antibody; CSBP2 antibody; CSPB1 antibody; Cytokine suppressive anti-inflammatory dr µg-binding protein antibody; EXIP antibody; MAP kinase 14 antibody; MAP kinase MXI2 antibody; MAP kinase p38 alpha antibody; MAPK 14 antibody; MAPK14 antibody; MAX interacting protein 2 antibody; MAX-interacting protein 2 antibody; Mitogen Activated Protein Kinase 14 antibody; Mitogen activated protein kinase p38 alpha antibody; Mitogen-activated protein kinase 14 antibody; Mitogen-activated protein kinase p38 alpha antibody; MK14_HUMAN antibody; Mxi 2 antibody; MXI2 antibody; p38 ALPHA antibody; p38 antibody; p38 MAP kinase antibody; p38 MAPK antibody; p38 mitogen activated protein kinase antibody; p38ALPHA antibody; p38alpha Exip antibody; PRKM14 antibody; PRKM15 antibody; RK antibody; SAPK2A antibody; Stress-activated protein kinase 2a antibody
Product Type : polyclonal Ab Antibody
Immunogen Species : Homo sapiens (Human)
UniProt ID : Q16539
Immunogen : Recombinant Human Mitogen-activated protein kinase 14 protein (2-258AA)
Raised in : Rabbit
Species Reactivity : Human
Tested Applications : ELISA
Background : Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK14 is one of the four p38 MAPKs which play an important role in the cascades of cell µLar responses evoked by extracell µLar stim µLi such as proinflammatory cytokines or physical stress leading to direct activation of transcription factors. Accordingly, p38 MAPKs phosphorylate a broad range of proteins and it has been estimated that they may have approximately 200 to 300 substrates each. Some of the targets are downstream kinases which are activated thro µgh phosphorylation and further phosphorylate additional targets. RPS6KA5/MSK1 and RPS6KA4/MSK2 can directly phosphorylate and activate transcription factors such as CREB1 ATF1 the NF-kappa-B isoform RELA/NFKB3, STAT1 and STAT3, but can also phosphorylate histone H3 and the nucleosomal protein HMGN1. RPS6KA5/MSK1 and RPS6KA4/MSK2 play important roles in the rapid induction of immediate-early genes in response to stress or mitogenic stim µLi, either by inducing chromatin remodeling or by recruiting the transcription machinery. On the other hand, two other kinase targets, MAPKAPK2/MK2 and MAPKAPK3/MK3, participate in the control of gene expression mostly at the post-transcriptional level, by phosphorylating ZFP36 (tristetraprolin) and ELAVL1 and by reg µLating EEF2K, which is important for the elongation of mRNA during translation. MKNK1/MNK1 and MKNK2/MNK2, two other kinases activated by p38 MAPKs, reg µLate protein synthesis by phosphorylating the initiation factor EIF4E2. MAPK14 interacts also with casein kinase II, leading to its activation thro µgh autophosphorylation and further phosphorylation of TP53/p53. In the cytoplasm, the p38 MAPK pathway is an important reg µLator of protein turnover. For example, CFLAR is an inhibitor of TNF-induced apoptosis whose proteasome-mediated degradation is reg µLated by p38 MAPK phosphorylation. In a similar way, MAPK14 phosphorylates the ubiquitin ligase SIAH2, reg µLating its activity towards EGLN3. MAPK14 may also inhibit the lysosomal degradation pathway of autophagy by interfering with the intracell µLar trafficking of the transmembrane protein ATG9. Another function of MAPK14 is to reg µLate the endocytosis of membrane receptors by different mechanisms that impinge on the small GTPase RAB5A. In addition, clathrin-mediated EGFR internalization induced by inflammatory cytokines and UV irradiation depends on MAPK14-mediated phosphorylation of EGFR itself as well as of RAB5A effectors. Ectodomain shedding of transmembrane proteins is reg µLated by p38 MAPKs as well. In response to inflammatory stim µLi, p38 MAPKs phosphorylate the membrane-associated metalloprotease ADAM17. Such phosphorylation is required for ADAM17-mediated ectodomain shedding of TGF-alpha family ligands, which res µLts in the activation of EGFR signaling and cell proliferation. Another p38 MAPK substrate is FGFR1. FGFR1 can be translocated from the extracell µLar space into the cytosol and nucleus of target cells, and reg µLates processes such as rRNA synthesis and cell growth. FGFR1 translocation requires p38 MAPK activation. In the nucleus, many transcription factors are phosphorylated and activated by p38 MAPKs in response to different stim µLi. Classical examples include ATF1 ATF2, ATF6, ELK1 PTPRH, DDIT3, TP53/p53 and MEF2C and MEF2A. The p38 MAPKs are emerging as important mod µLators of gene expression by reg µLating chromatin modifiers and remodelers. The promoters of several genes involved in the inflammatory response, such as IL6, IL8 and IL12B, display a p38 MAPK-dependent enrichment of histone H3 phosphorylation on \'Ser-10\' (H3S10ph) in LPS-stim µLated myeloid cells. This phosphorylation enhances the accessibility of the cryptic NF-kappa-B-binding sites marking promoters for increased NF-kappa-B recruitment. Phosphorylates CDC25B and CDC25C which is required for binding to 14-3-3 proteins and leads to initiation of a G2 delay after µLtraviolet radiation. Phosphorylates TIAR following DNA damage, releasing TIAR from GADD45A mRNA and preventing mRNA degradation. The p38 MAPKs may also have kinase-independent roles, which are tho µght to be due to the binding to targets in the absence of phosphorylation. Protein O-Glc-N-acylation catalyzed by the OGT is reg µLated by MAPK14, and, altho µgh OGT does not seem to be phosphorylated by MAPK14, their interaction increases upon MAPK14 activation induced by glucose deprivation. This interaction may reg µLate OGT activity by recruiting it to specific targets such as neurofilament H, stim µLating its O-Glc-N-acylation. Required in mid-fetal development for the growth of embryo-derived blood vessels in the labyrinth layer of the placenta. Also plays an essential role in developmental and stress-induced erythropoiesis, thro µgh reg µLation of EPO gene expression. Isoform MXI2 activation is stim µLated by mitogens and oxidative stress and only poorly phosphorylates ELK1 and ATF2. Isoform EXIP may play a role in the early onset of apoptosis. Phosphorylates S100A9 at \'Thr-113\'.
Clonality : polyclonal Ab
Isotype : IgG
Purification Method : >95%, Protein G purified
Conj µgate : Biotin
Buffer : Preservative : 0.03% Proclin 300
Constituents : 50% Glycerol, 0.01M PBS, PH 7.4
Form : Liquid
Stroage : Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Target Names : MAPK14
Research Areas : Cancer; Immunology; Signal transduction