GAPDH Monoclonal Antibody

Volume : 50 µL
Clone Number : 14C2F11
Aliases : GAPDH; G3PD; GAPD; MGC88685
Product Type : Tag/Control Antibodies
Immunogen Species :
UniProt ID :
Immunogen : Recombinant Human GAPDH protein (3-335AA)
Raised in : Mouse
Species Reactivity : Human, Rat, Rabbit
Tested Applications : ELISA, WB, IHC, IP, IF, FC; Recommended dilution : WB : 1 : 5000-1 : 1600000, IHC : 1 : 50-1 : 500, IF : 1 : 50-1 : 200, IP : 1µl-2µl, FC : 1 : 100-1 : 300
Background : Glyceraldehyde 3-phosphate dehydrogenase (GAPDH or G3PDH) is an enzyme of 37kDa that is consisdered as a cell µLar enzyme involved in glycolysis. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a pleiotropic enzyme that is overexpressed in apoptosis and in several human chronic pathologies. Its role as a mediator for cell death has also been highlighted. At the molec µLar level, sequential steps lead to nuclear translocation of GAPDH during cell death as follows : first, a catalytic cysteine in GAPDH (C150 in rat GAPDH) is S-nitrosylated by nitric oxide (NO) that is generated from inducible nitric oxide synthase (iNOS) and/or neuronal NOS (nNOS); second, the modified GAPDH becomes capable of binding with Siah1 an E3 ubiquitin ligase, and stabilizes it; third, the GAPDH-Siah protein complex translocates to the nucleus, dependent on Siah1’s nuclear localization signal, and degrades Siah1’s substrates in the nucleus, which res µLts in cytotoxicity. A recent report s µggests that GAPDH may be genetically associated with late-onset of Alzheimer’s disease.-deprenyl, which has originally been used as a monoamine oxidase inhibitor for Parkinson’s disease, binds to GAPDH and displays neuroprotective actions.
Clonality : Monoclonal
Isotype : IgG1
Purification Method : >95%, Protein G purified
Conj µgate : Non-conj µgated
Buffer : Preservative : 0.03% Proclin 300
Constituents : 50% Glycerol, 0.01M PBS, PH 7.4
Form : Liquid
Stroage : Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Target Names : GAPDH
Research Areas :