Bionesium(TM) Proteinase K

Technical Data
Appearance
White amorphous lyophilized powder
Protein purity
≥95%
Specific activity
≥30 U/mg Protein
Deoxyribonuclease activity
No activity of deoxyribonuclease was detected
Ribonuclease activity
No activity of Ribonuclease was detected
Enzymatic Properties
Source
Tritirachium album
Classification
EC 3.4.21.64
Molec µLar weight
29 kDa (SDS-PAGE)
Isoelectric point
7.81
Optimal pH
7.0-12.0 Both have high activity
Char1
Optimum temperature
65 ℃
Chart2
pH stability
pH 4.5-12.5 (25 ℃, 16 h)
Chart3
Thermal stability Stable below 50 ℃（pH 8.0, 30 min） Chart4
Activator
SDS, Urea
Inhibitor
Diisopropylfluorophosphate (DFIP)，Benzenes µLfonyl fluoride (PMSF)
Storage conditions
Dry powder can be stored at - 20 ℃ for a long time; After dissolving, it sho µLd be packed into proper volume,
stored at 2-8 ℃ for a short time, and stored at - 20 ℃ for a long time.
Use :
The virus consists of a protein shell and an internal nucleic acid. To amplify the nucleic acid by PCR, the shell protein must be destroyed first. The role of protease K is to break down the protein shell of the virus, releasing nucleic acid for easy detection.
1. Gene diagnosis kit；2. RNA and DNA Extraction Kit；3. Extract non protein components from tissues and degrade protein impurities. For example, the preparation of DNA vaccine；4.
Preparation of chromosome DNA by p µLse electrophoresis；5. Western blotting；6. R&D and mass
production of enzyme based glycated albumin reagents for in vitro diagnosis.