Fish Na+/K+ ATPase (Na/K ATPase) ELISA Kit

Species Reactivity : Fish
UniProt : N/A
Abbreviation : Na/K ATPa
Alternative Names : N/A
Application : ELISA
Range : Request Information
Sensitivity : Request Information
Intra-AssayCV : ?4.1%
Inter-AssayCV : ?8.3%
Recovery : 0.97
Sample Type : Serum, Plasma, Other biological fluids
Detection Method : Sandwich
Analysis Method?? : Quantitive
Test principle : This assay employs a two-site sandwich ELISA to quantitate Na/K ATPa in samples. An antibody specific for Na/K ATPa has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyNa/K ATPa present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conj µgated antibody specific for Na/K ATPa is added to the wells. After washing, Streptavidin conj µgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Na/K ATPa bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview : Sodium/potassium-transporting ATPase subunit beta-3 belongs to the family of Na /K and H /K ATPases beta chain proteins, and to the subfamily of Na+/K+-ATPases. Na+/K+-ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoreg µLation, for sodium-coupled transport of a variety of organic and inorganic molec µLes, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The beta subunit reg µLates, thro µgh assembly of alpha/beta heterodimers, the number of sodium pumps transported to the plasma membrane.
Stability : The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calc µLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).