Bovine Lipopolysaccharide binding protein (LBP) ELISA Kit

Species Reactivity : Bovine (Bos taurus; Cattle)
UniProt : Q2TBI0
Abbreviation : LBP
Alternative Names : MGC22233; LPS-binding protein|lipopolysaccharide-binding protein
Application : ELISA
Range : 0.625-40 ng/mL
Sensitivity : 0.288 ng/mL
Intra-AssayCV : ?4.6%
Inter-AssayCV : ?7.9%
Recovery : 1.08
Sample Type : Serum, Plasma, Other biological fluids
Detection Method : Sandwich
Analysis Method?? : Quantitive
Test principle : This assay employs a two-site sandwich ELISA to quantitate LBP in samples. An antibody specific for LBP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyLBP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conj µgated antibody specific for LBP is added to the wells. After washing, Streptavidin conj µgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LBP bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview : LPS-binding protein (LBP) is serum factor known to reg µLate the endotoxin-induced cell µLar immune response. Sepsis is a morbid condition induced by a toxin, the introduction or accum µLation of which is most commonly caused by infection or trauma. Sepsis-inducing toxins have been found associated with pathogenic bacteria, viruses, plants and venoms.Among the well described bacterial toxins are the endotoxins or lipopolysaccharides (LPS) of the gram-negative bacteria. Upon introduction of LPS into the blood it binds to lipopolysaccharide binding protein (LBP). LBP recognizes the lipid A region of LPS and forms high affinity complexes with both ro µgh and smooth form LPS. During the acute phase, LBP is syntheVolumed by hepatocytes, and reaches very high concentrations in serum.
Stability : The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calc µLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).