Bovine Estriol (E3) ELISA Kit

Species Reactivity : Bovine (Bos taurus; Cattle)
UniProt : N/A
Abbreviation : E3
Alternative Names : N/A
Application : ELISA
Range : 1.23-100 ng/mL
Sensitivity : 0.48 ng/mL
Intra-AssayCV : ?6.5%
Inter-AssayCV : ?8.0%
Recovery : 1.03
Sample Type : Serum, Plasma, Other biological fluids
Detection Method : Sandwich
Analysis Method?? : Quantitive
Test principle : This assay employs a two-site sandwich ELISA to quantitate E3 in samples. An antibody specific for E3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyE3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conj µgated antibody specific for E3 is added to the wells. After washing, Streptavidin conj µgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of E3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview : Estriol is one of the three main estrogens produced by the human body.Estrogens (U.S., otherwise oestrogens or œstrogens) are a group of steroid compounds, named for their importance in the estrous cycle, and functioning as the primary female sex hormone, their name comes from estrus (period of fertility for female mammals) gen = to generate. Estrogens are used as part of some oral contraceptives, in estrogen replacement therapy for postmenopausal women, and in hormone replacement therapy for transwomen. Like all steroid hormones, estrogens readily diffuse across the cell membrane. Once inside the cell, they bind to and activate estrogen receptors which in turn up-reg µLate the expression of many genes. Additionally, estrogens have been shown to activate a G protein-coupled receptor, GPR30.
Stability : The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calc µLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).