Bovine Prokineticin receptor 1 (PROKR1) ELISA Kit

Species Reactivity : Bovine (Bos taurus; Cattle)
UniProt : Q8SPN2
Abbreviation : PROKR1
Alternative Names : GPR73; GPR73a; PKR1; ZAQ; G protein-coupled receptor 73|G protein-coupled receptor ZAQ|OTTHUMP00000159967
Application : ELISA
Range : Request Information
Sensitivity : Request Information
Intra-AssayCV : ?4.5%
Inter-AssayCV : ?9.4%
Recovery : 0.96
Sample Type : Serum, Plasma, Other biological fluids
Detection Method : Sandwich
Analysis Method?? : Quantitive
Test principle : This assay employs a two-site sandwich ELISA to quantitate PROKR1 in samples. An antibody specific for PROKR1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPROKR1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conj µgated antibody specific for PROKR1 is added to the wells. After washing, Streptavidin conj µgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PROKR1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview : The prokineticin receptor is a G-protein coupled receptor which binds the peptide hormone prokineticin. These receptors mediate gastrointestinal smooth muscle contraction and angiogenesis. There are two variants each encoded by a different gene. Prokineticin receptor 1 (also known as PKR1 or GPR73A), is encoded by the PROKR1 gene. GPR73A is a receptor for prokineticin 1. It is a member of the GPCR family and is coupled to the G(q) subclass of heteromeric G proteins. Activation of the receptor leads to mobilization of calcium, stim µLation of phosphoinositide turnover and activation of p44/p42 mitogen-activated protein kinase. GPR73A expression has been reported in adrenal, peripheral blood leukocytes, brain, colon, pancreas, prostate, rectum, salivary gland, small intestine, spleen, stomach, testis, and thyroid.
Stability : The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calc µLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).