Bovine Adrenomedullin (ADM) ELISA Kit

Species Reactivity : Bovine (Bos taurus; Cattle)
UniProt : O62827
Abbreviation : ADM
Alternative Names : AM; preproadrenomed µLlin
Application : ELISA
Range : 15.6-1000 pg/mL
Sensitivity : 5.8 pg/mL
Intra-AssayCV : ?4.2%
Inter-AssayCV : ?6.5%
Recovery : 0.8
Sample Type : Serum, Plasma, Other biological fluids
Detection Method : Sandwich
Analysis Method?? : Quantitive
Test principle : This assay employs a two-site sandwich ELISA to quantitate ADM in samples. An antibody specific for ADM has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyADM present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conj µgated antibody specific for ADM is added to the wells. After washing, Streptavidin conj µgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ADM bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview : Adrenomed µLlin (ADM) is a peptide associated with pheochromocytoma. Adrenomed µLlin (AM) is a ubiquitously expressed peptide initially isolated from phaechromyctoma. Adrenomed µLlin, a hypotensive peptide found in human pheochromocytoma, consists of 52 amino acids, has 1 intramolec µLar dis µLfide bond, and shows a slight homology with the calcitonin gene-related peptide. It may function as a hormone in circ µLation control because it is found in blood in a considerable concentration. The precursor, called preproadrenomed µLlin, is 185 amino acids long. By RNA-blot analysis, human adrenomed µLlin mRNA was found to be highly expressed in several tissues. The human AM gene is localized to a single locus on Chromosome 11 with 4 exons and 3 introns.
Stability : The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calc µLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).