Bovine Synaptojanin 2 binding protein (SYNJ2BP) ELISA Kit

Species Reactivity : Bovine (Bos taurus; Cattle)
UniProt : Q3T0C9
Abbreviation : SYNJ2BP
Alternative Names : ARIP2; FLJ11271; FLJ41973; OMP25; activin receptor interacting protein 5
Application : ELISA
Range : Request Information
Sensitivity : Request Information
Intra-AssayCV : ?6.3%
Inter-AssayCV : ?10.3%
Recovery : 1.05
Sample Type : Serum, Plasma, Other biological fluids
Detection Method : Sandwich
Analysis Method?? : Quantitive
Test principle : This assay employs a two-site sandwich ELISA to quantitate SYNJ2BP in samples. An antibody specific for SYNJ2BP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySYNJ2BP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conj µgated antibody specific for SYNJ2BP is added to the wells. After washing, Streptavidin conj µgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SYNJ2BP bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview : The 3-prime region of the transcript is AU rich and contains m µLtiple mRNA instability repeats. The deduced 206-amino acid protein contains an N-terminal amphipathic helix, followed by a PDZ domain and a C-terminal transmembrane domain. Omp25 mRNA was widely expressed in rat tissues. Omp25 localized to the mitochondrial outer membrane via its C-terminal transmembrane region, with the PDZ domain facing the cytoplasm. Overexpression of Omp25 res µLted in perinuclear clustering of mitochondria in transfected cells, and this effect was mimicked by enforced expression of Synj2a on the mitochondrial outer membrane but not by a Synj2a mutant lacking the inositol-5 phosphatase domain.
Stability : The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calc µLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).