Bovine Transmembrane protein 55A (TMEM55A) ELISA Kit

Species Reactivity : Bovine (Bos taurus; Cattle)
UniProt : Q3SZ48
Abbreviation : TMEM55A
Alternative Names : DKFZp762O076; PtdIns-4;5-P(2) 4-phosphatase type II
Application : ELISA
Range : Request Information
Sensitivity : Request Information
Intra-AssayCV : ?5.6%
Inter-AssayCV : ?7.9%
Recovery : 0.89
Sample Type : Serum, Plasma, Other biological fluids
Detection Method : Sandwich
Analysis Method?? : Quantitive
Test principle : This assay employs a two-site sandwich ELISA to quantitate TMEM55A in samples. An antibody specific for TMEM55A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTMEM55A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conj µgated antibody specific for TMEM55A is added to the wells. After washing, Streptavidin conj µgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TMEM55A bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview : TMEM55A catalyzes the degradation of phosphatidylinositol 4,5-bisphosphate (PtdIns-4,5-P2) by removing the 4-phosphate.The deduced 300-amino acid protein contains a central Cx(5)R phosphatase catalytic motif and 2 transmembrane domains near its C terminus, a characteristic of lysosomal transmembrane proteins. RNA dot blot analysis detected expression in all tissues examined. Fluorescence-tagged TMEM55A localized with membrane markers of late endosomes or lysosomes.Ungewickell et al. (2005) assayed the phosphatase activity of TMEM55A syntheVolumed in insect cells and found that it specifically catalyzed removal of the 4-phosphate from PtdIns-4,5-P2. It showed no activity toward other phosphatidylinositol substrates or inositol phosphates tested.
Stability : The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calc µLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).