Bovine Transmembrane and ubiquitin like domain containing protein 1 (TMUB1) ELISA Kit
Species Reactivity : Bovine (Bos taurus; Cattle)
UniProt : Q3ZBI9
Abbreviation : TMUB1
Alternative Names : C7orf21; MGC5442; SB144;
Application : ELISA
Range : Request Information
Sensitivity : Request Information
Intra-AssayCV : ?4.2%
Inter-AssayCV : ?9.2%
Recovery : 0.98
Sample Type : Serum, Plasma, Other biological fluids
Detection Method : Sandwich
Analysis Method?? : Quantitive
Test principle : This assay employs a two-site sandwich ELISA to quantitate TMUB1 in samples. An antibody specific for TMUB1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTMUB1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conj µgated antibody specific for TMUB1 is added to the wells. After washing, Streptavidin conj µgated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TMUB1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview : Tmub1 encodes for a protein, HOPS/D µLP, containing transmembrane domains and a ubiquitin-like domain (UBL) Ubiquitin can be employed to modify proteins by either single or m µLtiple ubiquitin conj µgation to mark them for proteasomal destruction, endocytosis and binding to other proteins. Tmub1/HOPS was reported to facilitate the recycling of AMPA receptors into synaptic membrane in c µLtured primary neurons. Tmub1/HOPS was also found overexpressed during liver regeneration and is an essential constituent of centrosome assembly during cell cycles in c µLture. Like many UBL domain proteins, the physiological and molec µLar function of Tmub1 is far from being clear. The res µLting mice Tmub1were put thro µgh an extensive battery of assays to discover physiological and behavioral phenotypes.
Stability : The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calc µLated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).